What is a serial dilution and why is the technique used? Each successive standard uses a small portion of the previous standard, which is diluted by solvent to generate the next calibration standard in the series. The more evenly spaced the calibration standards are over this range, makes the results of the analysis more reliable.
What is the major disadvantage of microbial counts performed by methods other than the serial dilution-agar plate procedure? The serial dilution agar plate procedure only accounts for living viable cells while other methods count for both living and dead cells. What is the major disadvantage of direct microbial counts compared with the serial dilution agar plate procedures? Systematic error (also called systematic bias) is consistent, repeatable error associated with faulty equipment or a flawed experiment design. These dilutions can be done in microtiter plates or test tubes depending on the volumes of sample and diluent used. A small amount of serum or solute can be serially diluted by transferring aliquots to diluent. Serial dilution is a common technique used in many immunologic procedures.
The parallel dilution method propagates errors more severely than the serial dilutions technique. What is the main disadvantage of the parallel dilutions technique? The range of possible solution concentrations is limited. What is the main disadvantage of the parallel dilutions technique? Without proper training, the opportunity for error could be substantial, and the impact may vary from small to great. Dilution errors are those errors associated with the sample preparation from the time a sample is gathered until the time the organisms are either counted or identified. Also the higher the dilution is when doing variable counts, the more common it is for an error to be made when estimating the count.ĭilution error. What are some sources of error in the serial dilution/direct plate counting method? Some sources of error include inaccurate transferring will cause less accurate dispensing.
What are some sources of error in the serial dilution method?
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